Immunological Specificity and Mechanism of Action of IgG
نویسندگان
چکیده
Although observations have implied that lupus anticoagulants have immunologic specificity toward anionic phospholipids. this assumption has been directly demonstrated in only one patient with a monoclonal 1gM paraprotein. We tested the generality of this hypothesis directly by isolating five lgG lupus anticoagulants from patients with lupuslike syndromes and/or thrombosis. lgG lupus anticoagulant fractions were isolated free of other plasma proteins and free of contaminating phospholipid by adsorption to and elution from cardiolipin-cholesterol-dicetyl phosphate liposomes. followed by chromatography on protein ASepharose. Cardiolipin liposomes. but not phosphatidylcholine liposomes, were capable of removing all, or nearly all. lupus anticoagulant activity from patient plasma. The affinity-purified lgG preparations reacted with cardiolipin,
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تاریخ انتشار 2005